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The ''N''-end rule is a rule related to ubiquitination, discovered by Alexander Varshavsky and co-workers in 1986. The rule states that the ''N''-terminal amino acid of a protein determines its half-life (likelihood of being degraded). The rule applies to both eukaryotic and prokaryotic organisms, but with different strength. However, only rough estimations of protein half-life can be deduced from this 'rule', as N-terminal amino acid modification can lead to variability and anomalies, whilst amino acid impact can also change from organism to organism. Other degradation signals, known as degrons, can also be found in sequence. ==Relationships== ''N''-terminal residues - approximate half-life of proteins for S. cerevisiae〔 * Met, Gly, Ala, Ser, Thr, Val, Pro - > 20 hrs (stabilizing) * Ile, Glu - approx. 30 min (stabilizing) * Tyr, Gln - approx. 10 min (destabilizing) * Leu, Phe, Asp, Lys - approx. 3 min (destabilizing) * Arg - approx. 2 min (destabilizing) "N"-terminal residues - approximate half-life of proteins in mammalian systems * Val -> 100h * Met, Gly -> 30h * Pro -> 20h * Ile -> 20h * Thr -> 7.2h * Leu -> 5.5h * Ala -> 4.4h * His -> 3.5h * Trp -> 2.8h * Tyr -> 2.8h * Ser -> 1.9h * Asn -> 1.4h * Lys -> 1.3h * Cys -> 1.2h * Asp -> 1.1h * Phe -> 1.1h * Glu -> 1.0h * Arg -> 1.0h * Gln -> 0.8h 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「N-end rule」の詳細全文を読む スポンサード リンク
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